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Preparation :
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Produced from sera of rabbits immunized with highly pure recombinant Human ICAM-1. Anti-Human ICAM-1 specific antibody was purified by affinity chromatography and then biotinylated.
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Catalog #:
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500-P287Bt
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Source :
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Rabbits
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Formulation :
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A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
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Stability :
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The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8oC. Frozen aliquots are stable for at least 6 months when stored at -20oC. Avoid repeated freeze-thaw cycles.
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Reconstitution:
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Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
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Applications :
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Elisa
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Direct:To detect Human ICAM-1 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human ICAM-1.
Sandwich:To detect Human ICAM-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human ICAM-1 (500-P287) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human ICAM-1.
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Western Blot
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To detect Human ICAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human ICAM-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Country of Origin :
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USA
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Size A |
25µg |
Ł125 |
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Size B |
50µg |
Ł160 |
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Size C |
1mg |
Ł2250 |
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Special
Sizes:
Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request.
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Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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